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Sperm increased hds normal dfi

A formal semen analysis only provides information about the quantitative parameters of semen and does not address its qualitative properties. Recently, a new test called the sperm DNA integrity assay SDIA or sperm chromatin structure assay SCSA has been developed to diagnose cases of unidentified male infertility with a normal semen analysis. SCSA is a helpful tool for measuring clinically important properties of sperm nuclear chromatin integrity. In order to measure the level of sperm DNA damage, a method called flow cytometry is utilized, which involves staining of sperm with a DNA dye called acridine orange. If the sperm DNA is damaged, orange yellow to red light is observed and if there is no damage, green fluorescence is detected. The percentage of damaged sperm is calculated by the software and reported as two separate variables.
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About SCSA Diagnostics

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Sperm DNA Fragmentation - Causes, Symptoms And Treatments

Diagnosis of male infertility has mainly been based on the World Health Organization WHO manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing in fertility work-up and assisted reproductive techniques. During the last couple of decades, numerous sperm DNA integrity tests have been developed. These are claimed to be characterized by a lower intraindividual variation, less intralaboratory and interlaboratory variation and thus less subjective than the conventional sperm analysis. However, not all the sperm chromatin integrity tests have yet been shown to be of clinical value. So far, the test that has been found to have the most stable clinical threshold values in relation to fertility is the sperm chromatin structure assay SCSA , a flow cytometric test that measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ.
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Sperm DNA Fragmentation

Treatment of differentiated thyroid cancer usually consists of a total thyroidectomy followed by one or several courses of radioiodine I. We investigated effects of such treatment on sperm DNA in a patient with differentiated thyroid carcinoma. The patient, a year-old male with differentiated thyroid carcinoma treated by total thyroidectomy and radioiodine therapy, performed 6 semen samples in total, 3 for sperm banking and 3 for semen exploration, that were analysed for classic semen parameters. Sperm DFI was increased 3. Sperm banking should be recommended before treatment.
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